Application
Immunoprecipitation of PAI2 protein from U87-MG whole cell extracts using 5 μg of PAI2 antibody . Western blot analysis was performed using PAI2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
PAI2 antibody detects PAI2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAI2 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PAI2 antibody detects PAI2 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAI2 protein stained by PAI2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample (30 µg of whole cell lysate) A: JC 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using PAI-2 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PAI2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAI2 (serpin peptidase inhibitor, clade B (ovalbumin), member 2)Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PAI2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rabbit, Sheep, BovineRabbit (83%), Sheep (81%), Bovine (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HsT1201 antibody PAI antibody PAI-2 antibody PAI2 antibody PLANH2 antibody SERPINB2 antibody serpin B2 antibody urokinase inhibitor antibody placental plasminogen activator inhibitor antibody plasminogen activator inhibitor 2 antibody "plasminoge |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Secreted , extracellular space |
Application
Immunoprecipitation of PAI2 protein from U87-MG whole cell extracts using 5 μg of PAI2 antibody . Western blot analysis was performed using PAI2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
PAI2 antibody detects PAI2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAI2 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PAI2 antibody detects PAI2 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAI2 protein stained by PAI2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample (30 µg of whole cell lysate) A: JC 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using PAI-2 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: U87-MG 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 , JC , U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
