Application
Immunofluorescence staining of methanol-fixed Hela cells using PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421) Antibody (left) or the same antibody preincubated with blocking peptide| Product Name | PAK1/PAK2/PAK3 (phospho Thr423/Thr402/Thr421) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAK1/PAK2/PAK3 (Phospho Thr423/Thr402/Thr421)The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 423 /402/421 (R-S-T(p)-M-V) derived from Human PAK1/PAK2/PAK3. |
| Modification | p-Thr423/Thr402/Thr421 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-P |
| Suggested starting dilutions are as follows. IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC130000 MGC130001 PAKalpha PAK1 p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog yeast) alpha-PAK p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related) p65-PAK serine/threonine-protein kinase PAK 1 STE20 homolog yeast p21 protein (Cdc42/Rac |
|---|---|
| Target | PAK1/PAK2/PAK3 (Phospho Thr423/Thr402/Thr421) |
| Molecular Weight(MW) | 61-67(PAK2)68-74(PAK1/3) (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421) Antibody (left) or the same antibody preincubated with blocking peptide| Application Notes | Suggested starting dilutions are as follows. IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
