Application
Western blot analysis of extracts from HeLa cells, using PAK2 (Phospho-Ser192) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | PAK2 (phospho Ser192) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PAK2 (phospho Ser192)The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PAK2 around the phosphorylation site of Ser192. |
| Modification | p-Ser192 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:1000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | p21-activated kinase 2 PAK65 PAK2 Gamma-PAK EC2.7.11.1 p21activated kinase 2 S6/H4 kinase PAK-2 p21activated kinase2 p58 EC 2.7.11.1 GammaPAK PAKgamma PAK 2 Gamma PAK p21 activated kinase 2 PAK2 (phospho S192) PAK2 (phospho Ser192) PAK2 ( |
|---|---|
| Target | PAK2 |
| Molecular Weight(MW) | 58 kDa (note) |
| Tissue Specificity | PAK2 (Phospho-Ser192) Antibody detects endogenous levels of PAK2 only when phosphorylated at serine192. |
| Cellular Localization | Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane. |
Application
Western blot analysis of extracts from HeLa cells, using PAK2 (Phospho-Ser192) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:1000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
