Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP (cleaved Asp214) antibody diluted at 1:3000.Application
Untreated (–) and treated (+) HCT116 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 (cleaved Asp214) antibody diluted at 1:1000.Application
PARP1 (cleaved Asp214) antibody detects PARP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PARP1 protein stained by PARP1 (cleaved Asp214) antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | PARP (cleaved Asp214) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PARP1 (cleaved Asp214) (poly (ADP-ribose) polymerase 1)This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human PARP1 (cleaved Asp214). The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PARP ADPRT 1 PARP-1 poly (ADP-ribose) polymerase 1 PARP1 ARTD1 PPOL pADPRT-1 ADPRT1 ADPRT |
|---|---|
| Target | PARP1 (cleaved Asp214) |
| Molecular Weight(MW) | 113 kDa (note) |
| Cellular Localization | Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP (cleaved Asp214) antibody diluted at 1:3000.
Application
Untreated (–) and treated (+) HCT116 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 (cleaved Asp214) antibody diluted at 1:1000.
Application
PARP1 (cleaved Asp214) antibody detects PARP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PARP1 protein stained by PARP1 (cleaved Asp214) antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | 293T , HCT116 , HCT116 (30uM cisplatin treatment for 24hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
