Application
Western blot analysis of extracts from A549 cells, treated with etoposide 25uM 24h, using PARP (Cleaved-Asp214) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | PARP (cleaved Asp214) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PARP (cleaved Asp214)Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PARP. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PPOL pADPRT1 PARP PARP1 ADPRT1 ADPRT 1 ADPRT PARP (cleaved D214) PARP (cleaved Asp214) PARP cleaved D214 PARP cleaved Asp214 Cleaved PARP (D214) Cleaved PARP (Asp214) |
|---|---|
| Target | PARP |
| Molecular Weight(MW) | 24 kDa (note) |
| Tissue Specificity | PARP (Cleaved-Asp214) Antibody detects endogenous levels of fragment of activated PARP resulting from cleavage adjacent to Asp214. |
Application
Western blot analysis of extracts from A549 cells, treated with etoposide 25uM 24h, using PARP (Cleaved-Asp214) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
