| Product Name | PARP (cleaved) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PARP (cleaved)PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP's N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro. |
| Immunogen | Synthetic peptide correspond to the N-terminal residues of the catalytic domain of human PARP. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:100~1:500 ICC/IF:1:20~1:100 |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 142 pADPRT1 PARP-1 PARP cleaved PARP pADPRT-1 P09874 ADPRT1 PARP1 173870 ADPRT PPOL PARP 1 pADPRT 1 |
|---|---|
| Target | PARP (cleaved) |
| Tissue Specificity | This antibody recognizes only the large fragment of PARP (89 kDa) and does not react with full length PARP. Campthothecin treated Jurkat cell lysate can be used as a positive control. |
| Application Notes | WB:1:100~1:500 ICC/IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline containing 50% glycerol, 1% BSA, and 0.02% thimerosal |
