Application
A. Western blot analysis of (1) Jurkat cells and (2) Jurkat + Staurosporine using anti-PARP-1 (p116/p85) RabMAb (catalog #) 1:1000 dilution.| Product Name | PARP (p116/p85) antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [E78] to PARP (p116/p85)Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair . As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 . Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) . |
| Immunogen | A synthetic peptide corresponding to residues near to C-terminal residues of human PARP-1 was used as immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | E78 |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| For WB: Use at a dilution of 1:1,000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | pADPRT1 PARP-1 PARP pADPRT-1 ADPRT1 PARP1 ADPRT 1 ADPRT PPOL PARP 1 pADPRT 1 |
|---|---|
| Target | PARP (p116/p85) |
| Molecular Weight(MW) | 116 / 85 (note) |
| Tissue Specificity | The antibody should recognize both pro-form and p85 cleaved-form of PARP-1. |
Application
A. Western blot analysis of (1) Jurkat cells and (2) Jurkat + Staurosporine using anti-PARP-1 (p116/p85) RabMAb (catalog #) 1:1000 dilution.| Application Notes | For WB: Use at a dilution of 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
