Application
staining PARP in human tonsil by Immunohistochemistry (FFPE-sections).Application
Human Tonsil stained with anti-Poly ADP-Ribose Polymerase (PARP) antibody| Product Name | PARP antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PARP predilutedPARP is a 116 kDa nuclear protein which is strongly activated by DNA strand breaks. PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation. During apoptosis, ICE family members, such as caspase 3 and 7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC-P: Working dilution, ready to use for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ADPRT ADPRT 1 ADPRT1 PARP PARP-1 PPOL pADPRT-1 PARP 1 pADPRT 1 PARP1 pADPRT1 |
|---|---|
| Target | PARP |
Application
staining PARP in human tonsil by Immunohistochemistry (FFPE-sections).
Application
Human Tonsil stained with anti-Poly ADP-Ribose Polymerase (PARP) antibody| Positive Control | Raji cells, tonsil |
|---|---|
| Application Notes | IHC-P: Working dilution, ready to use for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide |
