Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PATZ1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using PATZ1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 B: Hela 7.5% SDS PAGE diluted at 1:1000| Product Name | PATZ1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PATZ1 (POZ (BTB) and AT hook containing zinc finger 1)The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PATZ1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rabbit, BovineMouse (100%), Rat (100%), Rabbit (99%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MAZR antibody PATZ antibody RIAZ antibody ZBTB19 antibody ZNF278 antibody ZSG antibody dJ400N23 antibody PATZ1 antibody zinc finger and BTB domain-containing protein 19 antibody "POZ- AT hook- and zinc finger-containing protein 1 antibody" zinc |
|---|---|
| Molecular Weight(MW) | 74 kDa (note) (5) |
| Cellular Localization | Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PATZ1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using PATZ1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 B: Hela 7.5% SDS PAGE diluted at 1:1000| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
