Application
WB analysis of various cell lysates (75 μg per lane) using PAX5 antibody at a dilution of 1:1000.Application
ICC/IF analysis of Ramos and negative control Jurkat cells using PAX5 antibody at a dilution of 1:100 (green). F-Actin (red) was stained with Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.Application
ICC/IF analysis of Raji cells with (right) or without (left) PAX5 antibody at a dilution of 1:100 (green). Nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.Application
FACS analysis of permeabilized Ramos cells using PAX5 antibody at a dilution of 1:50 (red) or PBS alone (black). 30,000 cells were acquired for analysis.Application
IHC-P analysis of human tonsil tissues with (right) or without (left) PAX5 antibody at a dilution of 1:200. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.Application
IHC-P analysis of mouse lymph node tissues with (right) or without (right) PAX5 antibody at a dilution of 1:100. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.Application
IP analysis was performed using 750 ug of Raji lysate and 5 ug of PAX5 antibody. The precipitate was detected by the same antibody with EasyBlot as the secondary antibody.| Product Name | PAX5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PAX5This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of trans |
| Immunogen | Purified internal fragment of human recombinant PAX5 expressed in E. coli |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PIPWB |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC-P:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | BSAP paired box 5 |
|---|---|
| Target | PAX5 |
Application
WB analysis of various cell lysates (75 μg per lane) using PAX5 antibody at a dilution of 1:1000.
Application
ICC/IF analysis of Ramos and negative control Jurkat cells using PAX5 antibody at a dilution of 1:100 (green). F-Actin (red) was stained with Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.
Application
ICC/IF analysis of Raji cells with (right) or without (left) PAX5 antibody at a dilution of 1:100 (green). Nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Application
FACS analysis of permeabilized Ramos cells using PAX5 antibody at a dilution of 1:50 (red) or PBS alone (black). 30,000 cells were acquired for analysis.
Application
IHC-P analysis of human tonsil tissues with (right) or without (left) PAX5 antibody at a dilution of 1:200. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.
Application
IHC-P analysis of mouse lymph node tissues with (right) or without (right) PAX5 antibody at a dilution of 1:100. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.
Application
IP analysis was performed using 750 ug of Raji lysate and 5 ug of PAX5 antibody. The precipitate was detected by the same antibody with EasyBlot as the secondary antibody.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC-P:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C |
| Storage Buffer | PBS with 1mg/ml BSA, 30% glycerol |
