Application
WB analysis of rat eye extract using a PAX6 antibody.Application
WB analysis of ovine retinal extract using a PAX6 antibody.Application
WB analysis of indicated cell lysates (50ug per lane) using PAX6 antibody at a dilution of 1:1000.Application
ICC/IF analysis of untreated or All-Trans-Retinoic Acid (ATRA) treated (10uM, 24 hours) NCCIT cells using PAX6 antibody at a dilution of 1:50 (green). F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342. Images were taken at 20X magnification.Application
IP analysis was performed on 500ug of 293T extract with 5ug of PAX6 antibody. The precipitated protein was detected by the same antibody at a dilution of 1:1000.| Product Name | PAX6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAX6PAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters’ anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein. |
| Immunogen | Synthetic peptide corresponding to residues C R(267) E E K L R N Q R R Q A S N T P S H I(285) of mouse PAX6. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| ICC/IF: 1:50: |
|
| Species Reactivity | HumanMouseRatPrimateSheep |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Pax6 paired box 6 |
|---|---|
| Target | PAX6 |
Application
WB analysis of rat eye extract using a PAX6 antibody.
Application
WB analysis of ovine retinal extract using a PAX6 antibody.
Application
WB analysis of indicated cell lysates (50ug per lane) using PAX6 antibody at a dilution of 1:1000.
Application
ICC/IF analysis of untreated or All-Trans-Retinoic Acid (ATRA) treated (10uM, 24 hours) NCCIT cells using PAX6 antibody at a dilution of 1:50 (green). F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342. Images were taken at 20X magnification.
Application
IP analysis was performed on 500ug of 293T extract with 5ug of PAX6 antibody. The precipitated protein was detected by the same antibody at a dilution of 1:1000.| Application Notes | ICC/IF: 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
