Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin (Phospho-Tyr118) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 3T3 cells untreated or treated with serum using Paxillin (Phospho-Tyr118) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin (Phospho-Tyr118) (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 3T3 cells untreated or treated with serum using Paxillin (Phospho-Tyr118)| Product Name | Paxillin (phospho Tyr118) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Paxillin (Phospho Tyr118)Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 118 (H-V-Y(p)-S-F) derived from Human Paxillin. |
| Modification | p-Tyr118 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ16691 PXN paxillin |
|---|---|
| Target | Paxillin (Phospho Tyr118) |
| Molecular Weight(MW) | 68 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin (Phospho-Tyr118) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 3T3 cells untreated or treated with serum using Paxillin (Phospho-Tyr118) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin (Phospho-Tyr118) (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 3T3 cells untreated or treated with serum using Paxillin (Phospho-Tyr118)| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
