Application
Immunofluorescence staining of methanol-fixed Hela cells using Paxillin(phospho-Tyr88) AntibodyApplication
Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Paxillin(phospho-Tyr88) Antibody| Product Name | Paxillin (phospho Tyr88) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Paxillin (Phospho Tyr88)An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 88 (P-V-Y(p)-G-S) derived from Human Paxillin. |
| Modification | p-Tyr88 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ16691 PXN paxillin |
|---|---|
| Target | Paxillin (Phospho Tyr88) |
| Molecular Weight(MW) | 68 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using Paxillin(phospho-Tyr88) Antibody
Application
Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Paxillin(phospho-Tyr88) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
