Application
Immunofluorescence staining of methanol-fixed Hela cells using Paxillin(Ab-88) AntibodyApplication
Western blot analysis of extracts from HUVEC cells using Paxillin(Ab-88) Antibody and the same antibody preincubated with blocking peptide| Product Name | Paxillin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PaxillinCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). |
| Immunogen | Peptide sequence around aa.86~90 (P-V-Y-G-S) derived from Human Paxillin. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ16691 PXN paxillin |
|---|---|
| Target | Paxillin |
| Molecular Weight(MW) | 68 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using Paxillin(Ab-88) Antibody
Application
Western blot analysis of extracts from HUVEC cells using Paxillin(Ab-88) Antibody and the same antibody preincubated with blocking peptide| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
