Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with PBK Antibody (C-term C300) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of PBK (arrow) using rabbit polyclonal PBK Antibody (C-term C300) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PBK gene (Lane 2)Application
PBK/TOPK Antibody (Mouse C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PBK/TOPK antibody detected the PBK/TOPK protein (arrow).| Product Name | PBK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PBK (C-term C300)This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 293~322 amino acids from the C-terminal region of human PBK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MAPKK-like protein kinase TLAK cello PBK TLAK celloriginated protein kinase T-LAK cell-originated protein kinase T-lymphokine-activated killer cell-originated protein kinase Nori3 Tlymphokineactivated killer celloriginated protein kinase Spermatoge |
|---|---|
| Target | PBK |
| Molecular Weight(MW) | 36.085 kDa (note) |
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with PBK Antibody (C-term C300) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of PBK (arrow) using rabbit polyclonal PBK Antibody (C-term C300) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PBK gene (Lane 2)
Application
PBK/TOPK Antibody (Mouse C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PBK/TOPK antibody detected the PBK/TOPK protein (arrow).| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
