Application
PCB antibody detects PCB protein at mitochondria and cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PCB antibody diluted at 1:100.Application
PCB antibody detects PCB protein by western blot analysis. Various whole cell extracts (30 μg) and Mouse tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PCB antibody diluted at a dilution of 1:1000.| Product Name | PCB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PCB (pyruvate carboxylase)This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PCB. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB:1:500~1:2000 IHC-P:1:50~1:200 |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rhesus Monkey, BovineRat (100%), Pig (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | pyruvate carboxylase PC |
|---|---|
| Target | PCB |
| Molecular Weight(MW) | 130 kDa (note) (3) |
| Cellular Localization | Mitochondrion matrix |
Application
PCB antibody detects PCB protein at mitochondria and cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PCB antibody diluted at 1:100.
Application
PCB antibody detects PCB protein by western blot analysis. Various whole cell extracts (30 μg) and Mouse tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PCB antibody diluted at a dilution of 1:1000.| Positive Control | 293T , A431 , HeLa , mouse liver |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC-P:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
