Application
Immunoprecipitation of PCB protein from HeLa whole cell extracts using 5 μg of PCB antibody . Western blot analysis was performed using PCB antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
PCB antibody detects PCB protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PCB antibody diluted at a dilution of 1:1000.Application
PCB antibody detects PCB protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: PCB protein stained by PCB antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
PCB antibody detects PCB protein at cytoplasm in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. PCB antibody diluted at 1:500.| Product Name | PCB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PCB (pyruvate carboxylase)This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PCB. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Pig, Chicken, Rhesus Monkey, BovinePig (97%), Chicken (84%), Rhesus Monkey (100%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PC pyruvate carboxylase |
|---|---|
| Target | PCB |
| Molecular Weight(MW) | 130 kDa (note) (3) |
| Cellular Localization | Mitochondrion matrix |
Application
Immunoprecipitation of PCB protein from HeLa whole cell extracts using 5 μg of PCB antibody . Western blot analysis was performed using PCB antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
PCB antibody detects PCB protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PCB antibody diluted at a dilution of 1:1000.
Application
PCB antibody detects PCB protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: PCB protein stained by PCB antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
PCB antibody detects PCB protein at cytoplasm in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. PCB antibody diluted at 1:500.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
