Application
PCCB antibody [N2C3] detects PCCB protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. PCCB antibody [N2C3] diluted at 1:500.Application
Immunofluorescence analysis of methanol-fixed Hep G2, using PCCB antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded Lung Ad Cancer PC14 xenograft, using PCCB( ) antibody at 1:100 dilution.Application
Sample (30 µg whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PCCB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PCCB (propionyl Coenzyme A carboxylase, beta polypeptide)The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PCCB. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Rhesus Monkey, BovineRat (91%), Zebrafish (90%), Xenopus laevis (90%), Pig (94%), Rhesus Monkey (99%), Bovine (97%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp451E113 antibody PCCB antibody propanoyl-CoA:carbon dioxide ligase subunit beta antibody "propionyl Coenzyme A carboxylase beta polypeptide antibody" PCCase subunit beta antibody "propionyl-CoA carboxylase beta chain mitochondrial antibody" "p |
|---|---|
| Molecular Weight(MW) | 58 kDa (note) (2) |
| Cellular Localization | Mitochondrion matrix |
Application
PCCB antibody [N2C3] detects PCCB protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. PCCB antibody [N2C3] diluted at 1:500.
Application
Immunofluorescence analysis of methanol-fixed Hep G2, using PCCB antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Lung Ad Cancer PC14 xenograft, using PCCB( ) antibody at 1:100 dilution.
Application
Sample (30 µg whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
