Application
IHC-P analysis of human spleen tissue using PCNA antibody at 4.0-8.0μg/ml.Application
WB analysis of HepG2 cells using PCNA antibody at 1.25μg/ml.Application
ICC/IF analysis of NT-2 cells using PCNA antibody at 1:500. Red : PCNA Blue : DAPIApplication
WB analysis of NT-2 cells using PCNA antibody at 2μg/ml.| Product Name | PCNA antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PCNAThe protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide corresponding to a C-terminal region of Human PCNA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Cow: 100%; Dog: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 91% |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | PCNA |
|---|---|
| Target | PCNA |
| Molecular Weight(MW) | 29 KDa (note) |
Application
IHC-P analysis of human spleen tissue using PCNA antibody at 4.0-8.0μg/ml.
Application
WB analysis of HepG2 cells using PCNA antibody at 1.25μg/ml.
Application
ICC/IF analysis of NT-2 cells using PCNA antibody at 1:500. Red : PCNA Blue : DAPI
Application
WB analysis of NT-2 cells using PCNA antibody at 2μg/ml.| Positive Control | HepG2 , NT-2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
