Application
Immunofluorescence analysis of methanol-fixed HeLa, using PDE10A antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PDE10A antibody at 1:500 dilution.Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PDE10A antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDE10A (phosphodiesterase 10A)Various cellular responses are regulated by the second messengers cAMP and cGMP. Phosphodiesterases, such as PDE10A, eliminate cAMP- and cGMP-mediated intracellular signaling by hydrolyzing the cyclic nucleotide to the corresponding nucleoside 5-prime monophosphate (Fujishige et al., 2000 [PubMed 10998054]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PDE10A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, ZebrafishMouse (98%), Rat (98%), Zebrafish (84%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ11894 antibody FLJ25677 antibody HSPDE10A antibody PDE10A antibody dJ416F21.1 (phosphodiesterase 10A) antibody "cAMP and cAMP-inhibited cGMP 3' 5'-cyclic phosphodiesterase 10A antibody" phosphodiesterase 10A1 (PDE10A1) antibody phosphodiesterase |
|---|---|
| Molecular Weight(MW) | 88 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PDE10A antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PDE10A antibody at 1:500 dilution.
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE10A antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HCT116 , Human PDE10A-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
