Application
Western blot of PC-PD11 with anti PD11 antibodies. 1. 1:500 dilution 2. 1:500 dilution| Product Name | PDE11A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDE11AOne of the many phosphodiesterases that compartmentalize and hydrolyze cAMP and cGMP into AMP and GMP is Phosphodiesterase type 11. The human PDE11A contains an open reading frame encoding a 490 amino acid protein (55-56 kDa). The PDE11A has an N-terminus GAF domain homologous to other signaling molecule as found in PDE2, PDE5, PDE6, PDE10, which potentially represents an allosteric cGMP and other small signaling molecules. PDE11A hydrolyzes both cGMP and cAMP; it is sensitive to non-selective PDE inhibitor IBMX as well as zaprinast and dipyrimadole inhibitors that are generally selective for cGMP-specific enzymes. PDE11A expression is diverse and is found in high levels in skeletal muscle, prostate, kidney, liver, pituitary and testis. |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to C terminal amino acids 454-468 of Human PDE11A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects bands corresponding to at least two PDE11A variants. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ11894 FLJ25677 HSPDE10A |
|---|---|
| Target | PDE11A |
| Tissue Specificity | This antibody does not cross-react with any of the members of the PDE family tested so far. |
Application
Western blot of PC-PD11 with anti PD11 antibodies. 1. 1:500 dilution 2. 1:500 dilution| Positive Control | Rat testis, skeletal muscle, kidney and brain |
|---|---|
| Application Notes | IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects bands corresponding to at least two PDE11A variants. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4oC short term. For extended storage aliquot and store at -20oC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Stabilization buffer' with 0.02% thimerosal or merthiolate. |
