Application
PDE4B antibody detects PDE4B protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE4B antibody diluted by 1:1000.Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 7.5% SDS PAGE diluted at 1:1000| Product Name | PDE4B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDE4B (phosphodiesterase 4B, cAMP-specific)This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PDE4B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineMouse (97%), Rat (96%), Xenopus laevis (83%), Pig (97%), Chicken (87%), Rhesus Monkey (100%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686F2182 antibody DPDE4 antibody MGC126529 antibody PDE4B5 antibody PDEIVB antibody PDE4B antibody "phosphodiesterase 4B cAMP-specific (phosphodiesterase E4 dunce homolog Drosophila) antibody" dunce-like phosphodiesterase E4 antibody "cAMP-s |
|---|---|
| Molecular Weight(MW) | 83 kDa (note) (7) |
Application
PDE4B antibody detects PDE4B protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PDE4B antibody diluted by 1:1000.
Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 7.5% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , PC-3 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
