Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PDE4D antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using PDE4D antibody at 1:100 dilution.Application
Sample (30 µg of whole cell lysate) A: A431 B: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PDE4D antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDE4D (phosphodiesterase 4D, cAMP-specific)The PDE4D gene is complex and has at least 9 different isoforms that encode functional proteins. These proteins degrade the second messenger cAMP, which is a key signal transduction molecule in multiple cell types, including vascular cells (Dominiczak and McBride, 2003 [PubMed 14517535]).[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PDE4D. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686M11213 antibody DPDE3 antibody FLJ97311 antibody HSPDE4D antibody PDE4DN2 antibody STRK1 antibody PDE4D antibody "phosphodiesterase 4D cAMP-specific (phosphodiesterase E3 dunce homolog Drosophila) antibody" cAMP-specific phosphodiesterase |
|---|---|
| Molecular Weight(MW) | 91 kDa (note) (9) |
| Cellular Localization | Cytoplasm (By similarity) , Membrane (By similarity) , Cytoplasm , cytoskeleton (By similarity) , Centrosome (By similarity) |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PDE4D antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using PDE4D antibody at 1:100 dilution.
Application
Sample (30 µg of whole cell lysate) A: A431 B: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
