Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using PDGFB antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:500| Product Name | PDGFB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDGFB (platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog))The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PDGFB. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (87%), Bovine (80%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ12858 antibody PDGF2 antibody SIS antibody SSV antibody c-sis antibody PDGFB antibody "Platelet-derived growth factor beta polypeptide (oncogene SIS) antibody" platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogen |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) (2) |
| Cellular Localization | Secreted |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using PDGFB antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:500| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
