Application
Immunofluorescence staining of methanol-fixed Hela cells using PDK1 (Phospho-Ser241) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1 (Phospho-Ser241) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 and PC12 cells untreated or treated with EGF using PDK1 (Phospho-Ser241) Antibody| Product Name | PDK1 (phospho Ser241) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDK1 (Phospho Ser241)Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development. |
| Immunogen | Peptide sequence around phosphorylation site of serine 241 (A-N-S(p)-F-V) derived from Human PDK1. |
| Modification | p-Ser241 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC20087 MGC35290 PDK1 PRO0461 PDPK1 PkB kinase like gene 1 3-phosphoinositide-dependent protein kinase 1 PkB-like 1 3-phosphoinositide dependent protein kinase-1 |
|---|---|
| Target | PDK1 (Phospho Ser241) |
| Molecular Weight(MW) | 63 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using PDK1 (Phospho-Ser241) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1 (Phospho-Ser241) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 and PC12 cells untreated or treated with EGF using PDK1 (Phospho-Ser241) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
