Application
IP ImageImmunoprecipitation of PDK1 protein from 293T whole cell extracts using 5 μg of PDK1 antibody . Western blot analysis was performed using PDK1 antibody . EasyBlot anti-Rabbit IgG (-01) was used as a secondary reagent.| Product Name | PDK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDK1 (pyruvate dehydrogenase kinase, isozyme 1)Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PDK1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Chicken, BovineRat (94%), Pig (95%), Chicken (83%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PDK1 antibody "pyruvate dehydrogenase kinase isoenzyme 1 antibody" "pyruvate dehydrogenase kinase isozyme 1 antibody" "mitochondrial pyruvate dehydrogenase lipoamide kinase isoenzyme 1 antibody" |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) (2) |
| Cellular Localization | Mitochondrion matrix |
Application
IP ImageImmunoprecipitation of PDK1 protein from 293T whole cell extracts using 5 μg of PDK1 antibody . Western blot analysis was performed using PDK1 antibody . EasyBlot anti-Rabbit IgG (-01) was used as a secondary reagent.| Positive Control | A431 , mouse heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
