Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HepG2, using PDK3 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using PDK3 antibody at 1:100 dilution.| Product Name | PDK3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDK3 (pyruvate dehydrogenase kinase, isozyme 3)Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PDK3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-P |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, BovineMouse (96%), Rat (96%), Chicken (93%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PDK3 antibody "pyruvate dehydrogenase kinase isozyme 3 antibody" "pyruvate dehydrogenase lipoamide kinase isozyme 3 mitochondrial antibody" "pyruvate dehydrogenase kinase isoenzyme 3 antibody" |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) |
| Cellular Localization | Mitochondrion matrix |
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HepG2, using PDK3 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using PDK3 antibody at 1:100 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
