Application
Western blot analysis of PDL-1 in Raji cell lysate with PDL-1 antibody at (A) 0.5 and (B) 1 ug/mL.Application
Immunohistochemistry of PDL-1 in human heart tissue with PDL-1 antibody at 2.5 ug/mL.Application
Immunofluorescence of PDL-1 in Human Heart cells with PDL-1 antibody at 20 ug/mL.Application
Flow cytometry analysis of A-20 cells using PDL-1 antibody at 0.5 ug/ml. Green: Isotype control. Red : PDL-1 antibody.| Product Name | PD-L1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PD-L1 / CD274 |
| Immunogen | The immunogen corresponding to a region within amino acids 51 and 100 of human PD-L1 (Accession number: NP_054862). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| Recommended Starting Dilutions:
For WB: Use at a concentration of 0.5 - 1 μg/mL.
For IHC-P: Use at a concentration of 2.5 μg/mL.
For ICC/IF: Use at a concentration of 20 μg/mL.
For FACS: Use at a concentration of 0.5 μg/ml.
Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD274 molecule B7H1 PD-L1 PDL1 PDCD1LG1 PDCD1L1 B7-H CD274 |
|---|---|
| Target | PD-L1 / CD274 |
Application
Western blot analysis of PDL-1 in Raji cell lysate with PDL-1 antibody at (A) 0.5 and (B) 1 ug/mL.
Application
Immunohistochemistry of PDL-1 in human heart tissue with PDL-1 antibody at 2.5 ug/mL.
Application
Immunofluorescence of PDL-1 in Human Heart cells with PDL-1 antibody at 20 ug/mL.
Application
Flow cytometry analysis of A-20 cells using PDL-1 antibody at 0.5 ug/ml. Green: Isotype control. Red : PDL-1 antibody.| Application Notes | Recommended Starting Dilutions:
For WB: Use at a concentration of 0.5 - 1 μg/mL.
For IHC-P: Use at a concentration of 2.5 μg/mL.
For ICC/IF: Use at a concentration of 20 μg/mL.
For FACS: Use at a concentration of 0.5 μg/ml.
Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4ºC for three months and -20ºC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
