Application
PER1 antibody immunoprecipitates PER1 protein in IP experiments. IP samples: HeLa nuclear extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of PER1 protein by 4 μg PER1 antibody 5 % SDS-PAGE The immunoprecipitated PER1 protein was detected by PER1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PER1 antibody diluted at 1:1000.Application
PER1 antibody detects PER1 protein by western blot analysis. A. 30 μg drosophila lysate/extract 5 % SDS-PAGE PER1 antibody dilution: 1:500Application
PER1 antibody detects PER1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PER1 protein stained by PER1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining.Application
PER1 antibody detects PER1 protein by western blot analysis. A. 30 μg Rat2 whole cell lysate/extract 5 % SDS-PAGE PER1 antibody dilution: 1:1000Application
PER1 antibody detects PER1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg HeLa whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE PER1 antibody dilution: 1:1000| Product Name | PER1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PER1 (period circadian clock 1)This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known. Alternative splicing has been observed in this gene; however, these variants have not been fully described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PER1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanDrosophilaRat |
| Predicted Reactivity | Mouse, BovineMouse (88%), Bovine (88%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | RIGUI PER period circadian clock 1 hPER |
|---|---|
| Target | PER1 |
| Molecular Weight(MW) | 136 kDa (note) |
| Cellular Localization | Nucleus (By similarity) , Cytoplasm (By similarity) |
Application
PER1 antibody immunoprecipitates PER1 protein in IP experiments. IP samples: HeLa nuclear extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of PER1 protein by 4 μg PER1 antibody 5 % SDS-PAGE The immunoprecipitated PER1 protein was detected by PER1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PER1 antibody diluted at 1:1000.
Application
PER1 antibody detects PER1 protein by western blot analysis. A. 30 μg drosophila lysate/extract 5 % SDS-PAGE PER1 antibody dilution: 1:500
Application
PER1 antibody detects PER1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PER1 protein stained by PER1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
PER1 antibody detects PER1 protein by western blot analysis. A. 30 μg Rat2 whole cell lysate/extract 5 % SDS-PAGE PER1 antibody dilution: 1:1000
Application
PER1 antibody detects PER1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg HeLa whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 5 % SDS-PAGE PER1 antibody dilution: 1:1000| Positive Control | 293T , HeLa , HepG2 , Drosophila , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
