Application
Immunofluorescence analysis of methanol-fixed HeLa, using PFKFB1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using PFKFB1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela 10% SDS PAGE diluted at 1:1000| Product Name | PFKFB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PFKFB1 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1)This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PFKFB1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, BovineMouse (83%), Rat (94%), Xenopus laevis (80%), Pig (98%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | F6PK antibody HL2K antibody MGC116715 antibody MGC116717 antibody PFRX antibody PFKFB1 antibody PFK/FBPase 1 antibody "fructose-6-phosphate 2-kinase:fructose-2 6-bisphosphatase antibody" "6PF-2-K/Fru-2 6-P2ase 1 antibody" "6PF-2-K/Fru-2 6-P2ASE li |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (2) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PFKFB1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using PFKFB1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , H1299 , HeLa , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
