Application
PFKL antibody [C1C3] detects PFKL protein at cytoplasm in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. PFKL antibody [C1C3] diluted at 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PFKL antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PFKL antibody at 1:500 dilution.| Product Name | PFKL antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PFKL (phosphofructokinase, liver)Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PFKL. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chicken, BovineRat (95%), Chicken (88%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686G1648 antibody DKFZp686L2097 antibody FLJ30173 antibody FLJ40909 antibody PFK-B antibody PFKL antibody liver-type 1-phosphofructokinase antibody phosphohexokinase antibody phosphofructokinase 1 antibody "6-phosphofructokinase liver type a |
|---|---|
| Molecular Weight(MW) | 85 kDa (note) (2) |
Application
PFKL antibody [C1C3] detects PFKL protein at cytoplasm in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. PFKL antibody [C1C3] diluted at 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PFKL antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PFKL antibody at 1:500 dilution.| Positive Control | A431 , H1299 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
