Application
Sample (30 µg of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PFKP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PFKP (phosphofructokinase, platelet)The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PFKP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Rabbit, BovineRat (88%), Xenopus laevis (81%), Rabbit (93%), Bovine (90%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ40226 antibody PFK-C antibody PFKF antibody PFKP antibody phosphohexokinase antibody phosphofructokinase 1 antibody phosphofructo-1-kinase isozyme C antibody "Phosphofructokinase platelet type antibody" 6-phosphofructokinase type C antibody "6 |
|---|---|
| Molecular Weight(MW) | 86 kDa (note) (12) |
Application
Sample (30 µg of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 , HeLa , Molt-4 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
