Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGAM1 antibody [N1C3] diluted at 1:1000.Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: JurKat D: Raji 12% SDS PAGE diluted at 1:1000| Product Name | PGAM1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PGAM1 (phosphoglycerate mutase 1 (brain))Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PGAM1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, Rhesus Monkey, Chimpanzee, BovineRat (99%), Xenopus laevis (84%), Chicken (92%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PGAM-B antibody PGAMA antibody PGAM1 antibody "phosphoglycerate mutase A nonmuscle form antibody" phosphoglycerate mutase isozyme B antibody phosphoglycerate mutase 1 antibody BPG-dependent PGAM 1 antibody phosphoglycerate mutase 1 (brain) antibody |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) (3) |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGAM1 antibody [N1C3] diluted at 1:1000.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: JurKat D: Raji 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , Jurkat , Raji , mouse brain , 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
