Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using PHACTR3 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: PC-3 7.5% SDS PAGE diluted at 1:500Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000| Product Name | PHACTR3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PHACTR3 (phosphatase and actin regulator 3)The protein encoded by this gene is associated with the nuclear scaffold in proliferating cells. It was found to bind to the catalytic subunit of protein phosphatase-1 (PP1) and inhibit PP1 activity, suggesting that this protein may function as a regulatory subunit of PP1. Alternative splicing at this locus results in several transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PHACTR3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, PigRat (99%), Xenopus laevis (96%), Pig (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | C20orf101 antibody H17739 antibody MGC117178 antibody SCAPIN1 antibody SCAPININ antibody PHACTR3 antibody scaffold-associated PP1-inhibiting protein antibody phosphatase and actin regulator 3 antibody "phosphatase and actin regulator 3 isoform 1 a |
|---|---|
| Molecular Weight(MW) | 63 kDa (note) (7) |
| Cellular Localization | Nucleus matrix |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using PHACTR3 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: PC-3 7.5% SDS PAGE diluted at 1:500
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000| Positive Control | PC-3 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
