Application
PHKA1 antibody detects PHKA1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PHKA1 antibody diluted by 1:500.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PHKA1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using PHKA1 antibody at 1:200 dilution.| Product Name | PHKA1 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PHKA1 (phosphorylase kinase, alpha 1 (muscle))The PHKA1 gene encodes the alpha subunit of muscle phosphorylase kinase (EC 2.7.1.38), a key regulatory enzyme of glycogen metabolism. Phosphorylase kinase consists of 4 copies of an alpha-beta-gamma-delta tetramer. The alpha, beta (PHKB; MIM 172490), and gamma (PHKG1; MIM 172470 and PHKG2; MIM 172471) subunits have several isoforms; the delta subunit is calmodulin (CALM1; MIM 114180). PHKA2 (MIM 306000) encodes the alpha subunit of liver-specific phosphorylase kinase and is also located on the X chromosome.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PHKA1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rabbit, Rhesus MonkeyMouse (92%), Rat (92%), Rabbit (96%), Rhesus Monkey (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC132604 antibody PHKA antibody PHKA1 antibody "phosphorylase kinase alpha 1 (muscle) muscle glycogenosis antibody" phosphorylase b kinase regulatory subunit alpha skeletal muscle isoform antibody "phosphorylase b kinase regulatory subunit alpha s |
|---|---|
| Molecular Weight(MW) | 137 kDa (note) (3) |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side |
Application
PHKA1 antibody detects PHKA1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PHKA1 antibody diluted by 1:500.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PHKA1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PHKA1 antibody at 1:200 dilution.| Positive Control | 293T , A431 , H1299 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
