Application
Western blot analysis of Phospholipase D2 (pY169) expression in HEK293T TNFa-treated (A); NIH3T3 TNFa-treated (B); H9C2 TNFa-treated (C) whole cell lysates.| Product Name | Phospholipase D2 (phospho Tyr169) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Phospholipase D2 (phospho Tyr169)The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Phospholipase D2. The exact sequence is proprietary. |
| Modification | p-Tyr169 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PLD2 phospholipase D2 |
|---|---|
| Target | Phospholipase D2 (phospho Tyr169) |
| Tissue Specificity | Recognizes endogenous levels of Phospholipase D2 (pY169) protein. |
Application
Western blot analysis of Phospholipase D2 (pY169) expression in HEK293T TNFa-treated (A); NIH3T3 TNFa-treated (B); H9C2 TNFa-treated (C) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
