Application
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using PI3 kinase p85 alpha antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Jurkat B: THP-1 7.5% SDS PAGE diluted at 1:2000| Product Name | PI3 kinase p85 alpha antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PI3 kinase p85 alpha (phosphatidylinositol 3-kinase regulatory subunit alpha)Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PI3 kinase p85 alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Rhesus Monkey, BovineMouse (100%), Rat (99%), Zebrafish (86%), Xenopus laevis (88%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GRB1 antibody p85 antibody p85-ALPHA antibody PIK3R1 antibody phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody "phosphatidylinositol 3-kinase regulatory subunit polypeptide 1 (p85 alpha) antibody" PI3K regulatory subunit alpha |
|---|---|
| Molecular Weight(MW) | 84 kDa (note) (4) |
Application
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using PI3 kinase p85 alpha antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Jurkat B: THP-1 7.5% SDS PAGE diluted at 1:2000| Positive Control | Jurkat , THP-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
