Application
A. Western blot analysis of anti- PI3-K p85 subunit alpha RabMAb (cat. # ) dilution 1:2000. A: A431 B: MCF-7 C: Raji| Product Name | PI3 kinase p85 alpha antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP380Y] to PI3 kinase p85 alpha (C-term)PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; the regulatory subunit p85 and the catalytic subunit p110 . In quiescent cells p85 maintains p110 in a low activity state. Upon growth factor stimulation, p85 directly interacts with phosphotyrosine residues of activated growth factor receptors or adaptor proteins leading to activation of p110. p85 exists in two isoforms: p85 , including two short forms termed p50 and p55/AS53 and p85 including short form p55 PIK/p55 . The p85 adaptor protein uses the inter-SH2 (iSH2) region to bind p110 and employs SH3 and SH2 domains, phosphotyrosine residues, and proline-rich motifs to interact with activated tyrosine kinases such as BCR/ABL and NPM/ALK or adaptor proteins including Shc, c-Cbl, Crk-L, and Gab2 . |
| Immunogen | A synthetic peptide corresponding to residues in the C-term of human PI3-K 85 alpha was used as an immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP380Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | p85 p85-ALPHA PIK3R1 p85ALPHA GRB1 PI3 kinase p85 subunit alpha PI3kinase p85 subunit alpha p85 ALPHA |
|---|---|
| Target | PI3 kinase p85 alpha (C-term) |
| Molecular Weight(MW) | 85 (note) |
Application
A. Western blot analysis of anti- PI3-K p85 subunit alpha RabMAb (cat. # ) dilution 1:2000. A: A431 B: MCF-7 C: Raji| Application Notes | Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
