Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody [C1C3] diluted at 1:500.Application
Immunoprecipitation of PI3-Kinase p150 protein from HeLa whole cell extracts using 5 μg of PI3-Kinase p150 antibody [C1C3] . Western blot analysis was performed using PI3-Kinase p150 antibody [C1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: Molt-4 B: Raji 7.5% SDS PAGE diluted at 1:1000| Product Name | PI3-Kinase p150 antibody [C1C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PIK3R4 (phosphoinositide-3-kinase, regulatory subunit 4)Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PI3-Kinase p150. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (98%), Rat (92%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC102700 antibody, VPS15 antibody, p150 antibody, PIK3R4 antibody, PI3-kinase p150 subunit antibody, "phosphoinositide-3-kinase, regulatory subunit 4, p150 antibody", phosphoinositide 3-kinase adaptor protein antibody, phosphatidylinositol 3-kinase-associ |
|---|---|
| Target | p150(PIK3R4) |
| Molecular Weight(MW) | 153 kDa (note) |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody [C1C3] diluted at 1:500.
Application
Immunoprecipitation of PI3-Kinase p150 protein from HeLa whole cell extracts using 5 μg of PI3-Kinase p150 antibody [C1C3] . Western blot analysis was performed using PI3-Kinase p150 antibody [C1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: Molt-4 B: Raji 7.5% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
