Application
Immunofluorescence analysis of methanol-fixed A431, using PI4 kinase alpha antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using PI4 kinase alpha antibody at 1:500 dilution.Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI4 kinase alpha antibody [C1C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PI4 kinase alpha antibody [C1C2], Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PI4 kinase alpha (phosphatidylinositol 4-kinase, catalytic, alpha)This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by this gene is a type III enzyme that is not inhibited by adenosine. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PI4 kinase alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, BovineMouse (98%), Rat (98%), Xenopus laevis (89%), Bovine (95%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ16556 antibody, PI4K-ALPHA antibody, PIK4CA antibody, pi4K230 antibody, PI4KA antibody, PI4-kinase alpha antibody, phosphatidylinositol 4-kinase 230 antibody, phosphatidylinositol 4-kinase alpha antibody, "phosphatidylinositol 4-kinase, type III, alpha |
|---|---|
| Molecular Weight(MW) | 231 kDa (note) (2) |
Application
Immunofluorescence analysis of methanol-fixed A431, using PI4 kinase alpha antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using PI4 kinase alpha antibody at 1:500 dilution.
Application
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI4 kinase alpha antibody [C1C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
