Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:1000| Product Name | PIGA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PIGA (phosphatidylinositol glycan anchor biosynthesis, class A)This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PIGA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Chicken, Rhesus MonkeyHuman (100%), Rat (83%), Chicken (80%), Rhesus Monkey (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GPI3 antibody, PIG-A antibody, PIGA antibody, phosphatidylinositol N-acetylglucosaminyltransferase subunit A antibody, GPI anchor biosynthesis antibody, GLCNAC-PI synthesis protein antibody, class A GlcNAc-inositol phospholipid assembly protein antibody, " |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein |
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
