Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000Application
Sample(30 ug whole cell lysate) A:293T 10% SDS PAGE diluted at 1:1000Application
PIGK antibody [N1C2] detects PIGK protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. PIGK antibody [N1C2] dilution: 1:500.| Product Name | PIGK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PIGK (phosphatidylinositol glycan anchor biosynthesis, class K)This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PIGK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Pig, Chicken, Rhesus Monkey, BovineRat (99%), Zebrafish (90%), Pig (98%), Chicken (95%), Rhesus Monkey (99%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GPI8 antibody, MGC22559 antibody, PIGK antibody, GPI8 homolog antibody, phosphatidylinositol-glycan biosynthesis class K protein antibody, PIG-K antibody, GPI transamidase subunit antibody, "phosphatidylinositol glycan, class K antibody", GPI-anchor transa |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein |
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000
Application
Sample(30 ug whole cell lysate) A:293T 10% SDS PAGE diluted at 1:1000
Application
PIGK antibody [N1C2] detects PIGK protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. PIGK antibody [N1C2] dilution: 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
