Application
PIN1 antibody detects PIN1 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 15 % SDS-PAGE PIN1 antibody dilution: 1:1000Application
PIN1 antibody detects PIN1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 15 % SDS-PAGE PIN1 antibody dilution: 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PIN1 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: Hep G2 B: Raji 12% SDS PAGE diluted at 1:1000| Product Name | PIN1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PIN1 (peptidylprolyl cis/trans isomerase, NIMA-interacting 1)The human PIN1 gene encodes an essential nuclear peptidylprolyl cis-trans isomerase (PPIase; EC 5.2.1.8) involved in regulation of mitosis. PIN1 belongs to a class of PPIases that includes the E. coli parvulin, yeast Ess1, and Drosophila dodo (dod) gene products (Lu et al., 1996 [PubMed 8606777]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PIN1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, BovineMouse (95%), Rat (97%), Xenopus laevis (85%), Pig (97%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DOD antibody, UBL5 antibody, PIN1 antibody, peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody, PPIase Pin1 antibody, protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1 antibody, rotamase Pin1 antibody, "peptidylprolyl cis/trans |
|---|---|
| Target | PIN1 |
| Molecular Weight(MW) | 18 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
PIN1 antibody detects PIN1 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 15 % SDS-PAGE PIN1 antibody dilution: 1:1000
Application
PIN1 antibody detects PIN1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 15 % SDS-PAGE PIN1 antibody dilution: 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PIN1 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 B: Raji 12% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
