Application
PINK1 antibody [N3C3] detects PINK1 protein at mitochondria by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: PINK1 protein stained by PINK1 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample(30 ug whole cell lysate) A:A431 7.5% SDS PAGE diluted at 1:1000| Product Name | PINK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PINK1 (PTEN induced putative kinase 1)This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PINK1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (84%), Bovine (88%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BRPK antibody, FLJ27236 antibody, PARK6 antibody, PINK1 antibody, PTEN-induced putative kinase protein 1 antibody, "serine/threonine-protein kinase PINK1, mitochondrial antibody", protein kinase BRPK antibody, PTEN induced putative kinase 1 antibody |
|---|---|
| Target | PINK1 |
| Molecular Weight(MW) | 63 kDa (note) (2) |
| Cellular Localization | Mitochondrion outer membrane; Single-pass membrane protein |
Application
PINK1 antibody [N3C3] detects PINK1 protein at mitochondria by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: PINK1 protein stained by PINK1 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample(30 ug whole cell lysate) A:A431 7.5% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
