Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 C: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed A549, using PIP5K1A antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using PIPK I alpha antibody at 1:100 dilution.| Product Name | PIP5K1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PIP5K1A (phosphatidylinositol-4-phosphate 5-kinase, type I, alpha) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PIP5K1A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Dog, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineRat (91%), Xenopus laevis (82%), Dog (95%), Pig (96%), Chicken (85%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PIP5K1A antibody, 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha antibody, ptdIns(4)P-5-kinase 1 alpha antibody, PIP5K1-alpha antibody, PIP5KIalpha antibody, phosphatidylinositol-4-phosphate 5-kinase type-1 alpha antibody, "phosphatidylinosi |
|---|---|
| Molecular Weight(MW) | 63 kDa (note) (2) |
| Cellular Localization | Cell membrane , Endomembrane system , Golgi apparatus , Golgi stack (By similarity) |
Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 C: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed A549, using PIP5K1A antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using PIPK I alpha antibody at 1:100 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
