Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PIP5K2 alpha antibody diluted at 1:1000.Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PIP5K2 alpha antibody diluted at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using PIP5K2 alpha antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 10% SDS PAGE diluted at 1:1000| Product Name | PIP5K2 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PIP5K2 alpha (phosphatidylinositol-5-phosphate 4-kinase type-2 alpha)Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PIP5K2 alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, BovineXenopus laevis (83%), Pig (98%), Chicken (96%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ13267 antibody, PI5P4KA antibody, PIP5K2A antibody, PIP5KII-alpha antibody, PIP5KIIA antibody, PIPK antibody, PIP4K2A antibody, PIP5KIII antibody, ptdIns(5)P-4-kinase isoform 2-alpha antibody, PI(5)P 4-kinase type II alpha antibody, "phosphatidylinosito |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) (2) |
Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PIP5K2 alpha antibody diluted at 1:1000.
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PIP5K2 alpha antibody diluted at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using PIP5K2 alpha antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 10% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
