Rabbit polyclonal antibody to Pirin (Pirinin (iron-binding nuclear protein))This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the center region of human Pirinin. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
*Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
Human
Predicted Reactivity
Mouse, Rat, Rhesus Monkey, BovineMouse (95%), Rat (95%), Rhesus Monkey (98%), Bovine (92%)
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
PIR antibody, probable quercetinase antibody, "probable quercetin 2,3-dioxygenase PIR antibody", pirin antibody, pirin (iron-binding nuclear protein) antibody
Target
Pirin
Molecular Weight(MW)
32 kDa (note) (3)
Cellular Localization
Nucleus
Application
Application
Sample (30 ug of whole cell lysate) A: A431 B: Hela 12% SDS PAGE diluted at 1:1000
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.