Application
Immunofluorescence analysis of methanol-fixed HeLa, using PKA 2 beta antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PKA 2 beta antibody at 1:500 dilution.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE diluted at 1:1000| Product Name | PKA 2 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKA 2 beta (protein kinase, cAMP-dependent, regulatory, type II, beta)cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PKA 2 beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, BovineRat (96%), Xenopus laevis (84%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRKAR2 antibody, RII-BETA antibody, PRKAR2B antibody, cAMP-dependent protein kinase type II-beta regulatory subunit antibody, cAMP-dependent protein kinase type II-beta regulatory chain antibody, H_RG363E19.2 antibody, WUGSC:H_RG363E19.2 antibody, "protein |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PKA 2 beta antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PKA 2 beta antibody at 1:500 dilution.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
