Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PKC alpha antibody at 1:500 dilution.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:2000Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody [C1C3] diluted at 1:5000.| Product Name | PKC alpha antibody [C1C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKC alpha (protein kinase C, alpha)Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PKC alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Rabbit, Chicken, Rhesus Monkey, BovineZebrafish (90%), Xenopus laevis (94%), Rabbit (100%), Chicken (98%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AAG6 antibody, MGC129900 antibody, MGC129901 antibody, PKC-alpha antibody, PKCA antibody, PRKACA antibody, PRKCA antibody, PKC-A antibody, protein kinase C alpha type antibody, aging-associated gene 6 antibody, "protein kinase C, alpha antibody" |
|---|---|
| Molecular Weight(MW) | 77 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PKC alpha antibody at 1:500 dilution.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:2000
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha antibody [C1C3] diluted at 1:5000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
