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PKC gamma antibody

Catalog Number:OM268730
OM268730
Discount Price
OM268730-100ul
1~2 Week
100ul
OM268730-50ul
50ul
OM268730-20ul
20ul
1~2 Week
Product Profile
Product Name PKC gamma antibody
Antibody Type Primary Antibodies
Product description Rabbit Polyclonal antibody to PKC gamma (protein kinase C, gamma)Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). [provided by RefSeq]
Immunogen Recombinant protein encompassing a sequence within the center region of human PKC gamma. The exact sequence is proprietary.
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications IHC-PWB
*Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity HumanMouseRat
Predicted Reactivity Rabbit, BovineRabbit (99%), Bovine (98%)
Concentration 1mg/ml (Please refer to the vial label for the specific concentration)
Purification Affinity purified
Target Information
Gene Synonyms MGC57564 antibody, PKC-gamma antibody, PKCC antibody, PKCG antibody, SCA14 antibody, PRKCG antibody, protein kinase C gamma type antibody, "protein kinase C, gamma antibody"
Molecular Weight(MW) 78 kDa (note) (3)
Application

Application

PKC gamma antibody detects PKC gamma protein by western blot analysis. A. 50 μg mouse brain extract 7.5 % SDS-PAGE PKC gamma antibody dilution: 1:1000

Application

PKC gamma antibody detects PKC gamma protein by western blot analysis. A. 50 μg rat brain extract 7.5 % SDS-PAGE PKC gamma antibody dilution: 1:1000

Application

PKC gamma antibody detects PKC gamma protein at cytoplasm on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PKC gamma antibody diluted at 1:500.

Application

Immunohistochemical analysis of paraffin-embedded Hun7 xenograft, using PKC gamma antibody at 1:500 dilution.

Application

Sample(30 ug whole cell lysate) A:293T 7.5% SDS PAGE diluted at 1:2000

Application

PKC gamma antibody detects PKC gamma protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. PKC gamma antibody diluted at 1:500.
Application Notes *Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
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